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Real‐time Tracking and Sensing of Cu + and Cu 2+ with a Single SERS Probe in the Live Brain: Toward Understanding Why Copper Ions Were Increased upon Ischemia
Author(s) -
Liu Jiaqi,
Liu Zhichao,
Wang Weikang,
Tian Yang
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202106193
Subject(s) - copper , ion , tracking (education) , ischemia , cusp (singularity) , electrochemistry , chemistry , analytical chemistry (journal) , electrode , cortex (anatomy) , materials science , neuroscience , biology , chromatography , medicine , psychology , pedagogy , geometry , mathematics , organic chemistry , cardiology
The imbalance of Cu + and Cu 2+ in the brain is closely related to neurodegenerative diseases. However, it still lacks of effective analytical methods for simultaneously determining the concentrations of Cu + and Cu 2+ . Herein, we created a novel SERS probe (CuSP) to real‐time track and accurately quantify extracellular concentrations of Cu + and Cu 2+ in the live brain. The present CuSP probe demonstrated specific ability for recognition of Cu + and Cu 2+ in a dual‐recognition mode. Then, a microarray consisting of 8 CuSP probes with high tempo‐spatial resolution and good accuracy was constructed for tracking and simultaneously biosensing of Cu + and Cu 2+ in the cerebral cortex of living brain. Using our powerful tool, it was found that that the concentrations of Cu 2+ and Cu + were increased by ≈4.26 and ≈1.80 times upon ischemia, respectively. Three routes were first discovered for understanding the mechanisms of the increased concentrations of Cu + and Cu 2+ during ischemia.