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Cover Picture: Local Deuteration Enables NMR Observation of Methyl Groups in Proteins from Eukaryotic and Cell‐Free Expression Systems (Angew. Chem. Int. Ed. 25/2021)
Author(s) -
Dubey Abhinav,
Stoyanov Nikolay,
Viennet Thibault,
Chhabra Sandeep,
Elter Shantha,
Borggräfe Jan,
Viegas Aldino,
Nowak Radosław P.,
Burdzhiev Nikola,
Petrov Ognyan,
Fischer Eric S.,
Etzkorn Manuel,
Gelev Vladimir,
Arthanari Haribabu
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202105426
Subject(s) - nuclear magnetic resonance spectroscopy , protein expression , chemistry , cover (algebra) , int , computational biology , biochemistry , combinatorial chemistry , stereochemistry , biology , computer science , mechanical engineering , engineering , operating system , gene
Methyl‐group NMR spectroscopy offers exceptional benefits for structural studies of complex biological systems. Yet, restrictive isotope labeling requirements have obstructed its use in emerging protein expression systems. In their Communication on page 13783, Manuel Etzkorn, Vladimir Gelev, Haribabu Arthanari, and co‐workers report a five‐step synthesis of stereoselectively methyl‐labeled leucine, which greatly facilitates applications in eukaryotic or cell‐free expression systems, providing desirable NMR sensors in so far inaccessible protein systems.