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Ultrasmall Copper (I) Sulfide Nanoparticles Prevent Hepatitis B Virus Infection
Author(s) -
Guo Xiao,
Sun Maozhong,
Gao Rui,
Qu Aihua,
Chen Chen,
Xu Chuanlai,
Kuang Hua,
Xu Liguang
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202103717
Subject(s) - hbcag , hbsag , hepatitis b virus , virology , virus , chemistry , antigen , in vitro , in vivo , biology , microbiology and biotechnology , biochemistry , immunology
Abstract Hepatitis B virus (HBV) poses a severe threat to public health and social development. Here, we synthesized 4±0.5 nm copper (I) sulfide (Cu 2 S) nanoparticles (NPs) with 46 mdeg chiroptical property at 530 nm to selectively cleavage HBV core antigen (HBcAg) and effectively blocked HBV assembly and prevented HBV infection both in vitro and in vivo under light at 808 nm. Experimental analysis showed that the chiral Cu 2 S NPs specific bound with the functional domain from phenylalanine 23 (F 23 ) to leucine 30 (L 30 ) from HBcAg primary sequence and the cutting site was between amino acid residues F 24 and proline 25 (P 25 ). Under excitation at 808 nm, the intracellular HBcAg concentration was reduced by 95 %, and in HBV transgenic mice, the levels of HBV surface antigen (HBsAg) and HBV DNA were decreased by 93 % and 86 %, respectively. Together, these results reveal the potential nanomedicine for HBV control and provide fresh tools for viral infection.