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Ratiometric Fluorescent Lateral Flow Immunoassay for Point‐of‐Care Testing of Acute Myocardial Infarction
Author(s) -
Wang Jing,
Jiang Chenxing,
Jin Jiening,
Huang Liang,
Yu Wenbo,
Su Bin,
Hu Jun
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202103458
Subject(s) - detection limit , immunoassay , fluorescence , point of care , myocardial infarction , naked eye , colloidal gold , materials science , chemistry , nanoparticle , chromatography , nanotechnology , antibody , cardiology , medicine , pathology , optics , physics , immunology
Abstract We report the development of a highly sensitive ratiometric fluorescent lateral flow immunoassay (RFLFIA) strip for rapid and accurate detection of acute myocardial infarction biomarker, namely heart‐type fatty acid binding protein (H‐FABP). The RFLFIA strip works in terms of ratiometric change of fluorescence signal, arising from blending of fluorescence emitted by two composite nanostructures conjugated to capture and probe antibodies and inner filter effect of gold nanoparticles. In conjunction with using custom smartphone‐based analytical device and tonality analysis, quantitative detection of H‐FABP was achieved with a low limit of detection at 0.21 ng mL −1 . The RFLFIA strip can generate a visually distinguishable green‐to‐red color change around the threshold concentration of H‐FABP (6.2 ng mL −1 ), thus allowing the semi‐quantitative diagnosis by the naked eye.