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Protein–Antibody Conjugates (PACs): A Plug‐and‐Play Strategy for Covalent Conjugation and Targeted Intracellular Delivery of Pristine Proteins
Author(s) -
Liu Bin,
Singh Khushboo,
Gong Shuai,
Canakci Mine,
Osborne Barbara A.,
Thayumanavan S.
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202103106
Subject(s) - conjugate , drug delivery , antibody , targeted drug delivery , chemistry , computational biology , antigen , nanotechnology , biology , materials science , immunology , mathematical analysis , mathematics
We report here on protein–antibody conjugates (PACs) that are used for antibody‐directed delivery of protein therapeutics to specific cells. PACs have the potential to judiciously combine the merits of two prolific therapeutic approaches—biologics and antibody–drug conjugates. We utilize spherical polymer brushes to construct PACs using the combination of two simple and efficient functionally orthogonal click chemistries. In addition to the synthesis and characterization of these nanoparticles, we demonstrate that PACs are indeed capable of specifically targeting cells based on the presence of target antigen on the cell surface to deliver proteins. The potentially broad adaptability of PACs opens up new opportunities for targeted biologics in therapeutics and sensing.