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Metal Complex Lipids for Fluid–Fluid Phase Separation in Coassembled Phospholipid Membranes
Author(s) -
Ohtani Ryo,
Anegawa Yuka,
Watanabe Hikaru,
Tajima Yutaro,
Kinoshita Masanao,
Matsumori Nobuaki,
Kawano Kenichi,
Yanaka Saeko,
Kato Koichi,
Nakamura Masaaki,
Ohba Masaaki,
Hayami Shinya
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202102774
Subject(s) - phospholipid , membrane , chemistry , phase (matter) , vesicle , crystallography , metal , fluorescence , analytical chemistry (journal) , chromatography , organic chemistry , biochemistry , optics , physics
We demonstrate a fluid–fluid phase separation in 1,2‐dimyristoyl‐ sn ‐glycero‐3‐phosphocholine (DMPC) membranes using a metal complex lipid of type [Mn(L1)] ( 1 ; HL1=1‐(2‐hydroxybenzamide)‐2‐(2‐hydroxy‐3‐formyl‐5‐hexadecyloxybenzylideneamino)ethane). Small amount of 1 produces two separated domains in DMPC, whose phase transition temperatures of lipids ( T c ) are both lower than that of the pristine DMPC. Variable temperature fluorescent microscopy for giant‐unilamellar vesicles of DMPC/ 1 hybrids demonstrates that visible phase separations remain in fluid phases up to 37 °C, which is clearly over the T c of DMPC. This provides a new dimension for the application of metal complex lipids toward controlling lipid distributions in fluid membranes.