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Time‐Resolved and Comprehensive Analysis of Surface Glycoproteins Reveals Distinct Responses of Monocytes and Macrophages to Bacterial Infection
Author(s) -
Suttapitugsakul Suttipong,
Tong Ming,
Wu Ronghu
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202102692
Subject(s) - glycoprotein , monocyte , microbiology and biotechnology , macrophage , immunology , biology , chemistry , biochemistry , in vitro
Glycoproteins on the surface of immune cells play extremely important roles in response to pathogens. Yet, a systematic and time‐resolved investigation of surface glycoproteins during the immune response remains to be explored. Integrating selective enrichment of surface glycoproteins with multiplexed proteomics, we globally and site‐specifically quantified the dynamics of surface glycoproteins on THP‐1 monocytes and macrophages in response to bacterial infection and during the monocyte‐to‐macrophage differentiation. The time‐resolved analysis reveals transient changes and differential remodeling of surface glycoproteins on both cell types, and potential upstream regulators and downstream effects of the regulated glycoproteins. Besides, we identified novel surface glycoproteins participating in the immune response such as APMAP, and site‐specific changes of glycoproteins. This study provides unprecedented information to deepen our understanding of glycoproteins and cellular activities.