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Design of an In‐Cell Protein Crystal for the Environmentally Responsive Construction of a Supramolecular Filament
Author(s) -
Abe Satoshi,
Pham Thuc Toan,
Negishi Hashiru,
Yamashita Keitaro,
Hirata Kunio,
Ueno Takafumi
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202102039
Subject(s) - covalent bond , supramolecular chemistry , protein crystallization , protein filament , chemistry , crystallization , molecule , nanotechnology , crystallography , materials science , crystal structure , biochemistry , organic chemistry
Protein assemblies can be designed for development of nano–bio materials. This has been achieved by modulating protein–protein interactions. However, fabrication of highly ordered protein assemblies remains challenging. Protein crystals, which have highly ordered arrangements of protein molecules, provide useful source matrices for synthesizing artificial protein assemblies. Here, we describe construction of a supramolecular filament structure by engineering covalent and non‐covalent interactions in a protein crystal. Performing in‐cell crystallization of Trypanosoma brucei cysteine protease cathepsin B (TbCatB), we achieved a precise arrangement of protein molecules while suppressing random aggregation due to disulfide bonds. We succeeded in synthesizing bundled filament from the crystals by autoxidation of cysteinyl thiols after the isolation of the crystals from living cells.