Premium
Synthesis, Characterization, and Nanodisc Formation of Non‐ionic Polymers **
Author(s) -
Ravula Thirupathi,
Ramamoorthy Ayyalusamy
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202101950
Subject(s) - nanodisc , polymer , chemistry , amphiphile , ionic liquid , surface modification , ionic bonding , divalent , membrane , drug delivery , nanotechnology , biophysics , lipid bilayer , materials science , ion , biochemistry , organic chemistry , copolymer , biology , catalysis
Although lipid nanodiscs are increasingly used in the structural studies of membrane proteins, drug delivery and other applications, the interaction between the nanodisc belt and the protein to be reconstituted is a major limitation. To overcome this limitation and to further broaden the scope of nanodiscs, a family of non‐ionic amphiphilic polymers synthesized by hydrophobic functionalization of fructo‐oligosaccharides/inulin is reported. We show the stability of lipid nanodiscs formed by these polymers against pH and divalent metal ions, and their magnetic‐alignment properties. The reported results also demonstrate that the non‐ionic polymers extract membrane proteins with unprecedented efficiency.