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Reversible Supra‐Folding of User‐Programmed Functional DNA Nanostructures on Fuzzy Cationic Substrates
Author(s) -
Nakazawa Koyomi,
El Fakih Farah,
Jallet Vincent,
RossiGendron Caroline,
Mariconti Marina,
Chocron Léa,
Hishida Mafumi,
Saito Kazuya,
Morel Mathieu,
Rudiuk Sergii,
Baigl Damien
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202101909
Subject(s) - polyelectrolyte , dna origami , folding (dsp implementation) , cationic polymerization , nanostructure , nanotechnology , substrate (aquarium) , materials science , self assembly , dna , chemistry , adsorption , crystallography , polymer , polymer chemistry , biochemistry , engineering , electrical engineering , composite material , oceanography , organic chemistry , geology
Abstract We report that user‐defined DNA nanostructures, such as two‐dimensional (2D) origamis and nanogrids, undergo a rapid higher‐order folding transition, referred to as supra‐folding, into three‐dimensional (3D) compact structures (origamis) or well‐defined μm‐long ribbons (nanogrids), when they adsorb on a soft cationic substrate prepared by layer‐by‐layer deposition of polyelectrolytes. Once supra‐folded, origamis can be switched back on the surface into their 2D original shape through addition of heparin, a highly charged anionic polyelectrolyte known as an efficient competitor of DNA‐polyelectrolyte complexation. Orthogonal to DNA base‐pairing principles, this reversible structural reconfiguration is also versatile; we show in particular that 1) it is compatible with various origami shapes, 2) it perfectly preserves fine structural details as well as site‐specific functionality, and 3) it can be applied to dynamically address the spatial distribution of origami‐tethered proteins.