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Formate Dehydrogenases Reduce CO 2 Rather than HCO 3 − : An Electrochemical Demonstration
Author(s) -
Meneghello Marta,
Oliveira Ana Rita,
JacqBailly Aurore,
Pereira Inês A. C.,
Léger Christophe,
Fourmond Vincent
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202101167
Subject(s) - formate , substrate (aquarium) , electrochemistry , formate dehydrogenase , chemistry , inorganic chemistry , combinatorial chemistry , biochemistry , catalysis , electrode , biology , ecology
Mo/W formate dehydrogenases catalyze the reversible reduction of CO 2 species to formate. It is thought that the substrate is CO 2 and not a hydrated species like HCO 3 − , but there is still no indisputable evidence for this, in spite of the extreme importance of the nature of the substrate for mechanistic studies. We devised a simple electrochemical method to definitively demonstrate that the substrate of formate dehydrogenases is indeed CO 2 .