A Palette of Minimally Tagged Sucrose Analogues for Real‐Time Raman Imaging of Intracellular Plant Metabolism | Zendy

Moliner Fabio | Zendy; Knox Kirsten | Zendy; Gordon Doireann | Zendy; Lee Martin | Zendy; Tipping William J. | Zendy; Geddis Ailsa | Zendy; Reinders Anke | Zendy; Ward John M. | Zendy; Oparka Karl | Zendy; Vendrell Marc | Zendy

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A Palette of Minimally Tagged Sucrose Analogues for Real‐Time Raman Imaging of Intracellular Plant Metabolism

Author(s) -

Moliner Fabio,

Knox Kirsten,

Gordon Doireann,

Lee Martin,

Tipping William J.,

Geddis Ailsa,

Reinders Anke,

Ward John M.,

Oparka Karl,

Vendrell Marc

Publication year - 2021

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.202016802

Subject(s) - sucrose , raman spectroscopy , intracellular , fructose , chemistry , metabolism , biochemistry , biophysics , biology , optics , physics

Sucrose is the main saccharide used for long‐distance transport in plants and plays an essential role in energy metabolism; however, there are no analogues for real‐time imaging in live cells. We have optimised a synthetic approach to prepare sucrose analogues including very small (≈50 Da or less) Raman tags in the fructose moiety. Spectroscopic analysis identified the alkyne‐tagged compound 6 as a sucrose analogue recognised by endogenous transporters in live cells and with higher Raman intensity than other sucrose derivatives. Herein, we demonstrate the application of compound 6 as the first optical probe to visualise real‐time uptake and intracellular localisation of sucrose in live plant cells using Raman microscopy.

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