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Cover Picture: Engineered SAM Synthetases for Enzymatic Generation of AdoMet Analogs with Photocaging Groups and Reversible DNA Modification in Cascade Reactions (Angew. Chem. Int. Ed. 1/2021)
Author(s) -
Michailidou Freideriki,
Klöcker Nils,
Cornelissen Nicolas V.,
Singh Rohit K.,
Peters Aileen,
Ovcharenko Anna,
Kümmel Daniel,
Rentmeister Andrea
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202015604
Subject(s) - cascade , methyltransferase , dna , enzyme , chemistry , stereochemistry , methionine , combinatorial chemistry , biochemistry , amino acid , methylation , chromatography
Sequence‐specific modification (“writing”) and light‐triggered removal (“erasing”) of DNA is reported for the first time in an enzymatic cascade. In their Research Article on page 480, Andrea Rentmeister and co‐workers engineered SAM synthetases suitable for converting methionine analogs with photo‐cleavable groups and show their compatibility with methyltransferases to provide DNA with a light‐sensitive “cage”. Crystal structure analysis revealed how substitutions in the active site led to accommodation of the photo‐cleavable group.