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Fluorescent and Water Dispersible Single‐Chain Nanoparticles: Core–Shell Structured Compartmentation
Author(s) -
Hoffmann Justus F.,
Roos Andreas H.,
Schmitt FranzJosef,
Hinderberger Dariush,
Binder Wolfgang H.
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202015179
Subject(s) - electron paramagnetic resonance , amphiphile , nanoparticle , azide , fluorescence , electron paramagnetic resonance spectroscopy , polymer , materials science , covalent bond , chemical engineering , nanotechnology , copolymer , photochemistry , chemistry , polymer chemistry , organic chemistry , nuclear magnetic resonance , physics , composite material , quantum mechanics , engineering
Single‐chain nanoparticles (SCNPs) are highly versatile structures resembling proteins, able to function as catalysts or biomedical delivery systems. Based on their synthesis by single‐chain collapse into nanoparticular systems, their internal structure is complex, resulting in nanosized domains preformed during the crosslinking process. In this study we present proof of such nanocompartments within SCNPs via a combination of electron paramagnetic resonance (EPR) and fluorescence spectroscopy. A novel strategy to encapsulate labels within these water dispersible SCNPs with hydrodynamic radii of ≈5 nm is presented, based on amphiphilic polymers with additional covalently bound labels, attached via the copper catalyzed azide/alkyne “click” reaction (CuAAC). A detailed profile of the interior of the SCNPs and the labels’ microenvironment was obtained via electron paramagnetic resonance (EPR) experiments, followed by an assessment of their photophysical properties.