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Singlet‐Contrast Magnetic Resonance Imaging: Unlocking Hyperpolarization with Metabolism **
Author(s) -
Eills J.,
Cavallari E.,
Kircher R.,
Di Matteo G.,
Carrera C.,
Dagys L.,
Levitt M. H.,
Ivanov K. L.,
Aime S.,
Reineri F.,
Münnemann K.,
Budker D.,
Buntkowsky G.,
Knecht S.
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202014933
Subject(s) - hyperpolarization (physics) , spin isomers of hydrogen , chemistry , singlet state , nuclear magnetic resonance , magnetic resonance imaging , polarization (electrochemistry) , proton , nuclear magnetic resonance spectroscopy , hydrogen , excited state , atomic physics , stereochemistry , physics , medicine , radiology , organic chemistry , quantum mechanics
Abstract Hyperpolarization‐enhanced magnetic resonance imaging can be used to study biomolecular processes in the body, but typically requires nuclei such as 13 C, 15 N, or 129 Xe due to their long spin‐polarization lifetimes and the absence of a proton‐background signal from water and fat in the images. Here we present a novel type of 1 H imaging, in which hyperpolarized spin order is locked in a nonmagnetic long‐lived correlated (singlet) state, and is only liberated for imaging by a specific biochemical reaction. In this work we produce hyperpolarized fumarate via chemical reaction of a precursor molecule with para ‐enriched hydrogen gas, and the proton singlet order in fumarate is released as antiphase NMR signals by enzymatic conversion to malate in D 2 O. Using this model system we show two pulse sequences to rephase the NMR signals for imaging and suppress the background signals from water. The hyperpolarization‐enhanced 1 H‐imaging modality presented here can allow for hyperpolarized imaging without the need for low‐abundance, low‐sensitivity heteronuclei.