Premium
Drug‐Sponge Lipid Nanocarrier for in Situ Cargo Loading and Release Using Dynamic Covalent Chemistry
Author(s) -
Liu Fei,
Niko Yosuke,
Bouchaala Redouane,
Mercier Luc,
Lefebvre Olivier,
Andreiuk Bohdan,
Vandamme Thierry,
Goetz Jacky G.,
Anton Nicolas,
Klymchenko Andrey
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202014259
Subject(s) - nanocarriers , prodrug , chemistry , covalent bond , drug delivery , doxorubicin , drug , cytotoxicity , controlled release , combinatorial chemistry , hydrazide , nanotechnology , biophysics , organic chemistry , biochemistry , materials science , pharmacology , in vitro , medicine , surgery , chemotherapy , biology
Currently, drug‐delivery strategies using nanocarriers (NCs) deal with encapsulation of cargo or its covalently modified prodrug. Herein, we propose a concept of reversible pH‐controlled capture and delivery of active cargo based on dynamic covalent chemistry inside lipid nano‐droplets (nanoemulsions), coined as “drug sponge”. We designed a highly lipophilic hydrazide (LipoHD) capable of reacting with a free cargo‐ketone (fluorescent dye and doxorubicin drug) directly inside lipid NCs, yielding a lipophilic hydrazone prodrug efficiently captured in the oil core. LipoHD‐loaded NCs spontaneously accumulated cargo‐ketones, yielding formulations stable against cargo leakage at pH 7.4, and further released their dye/drug cargo at low pH range (5.0–6.8) in solution and live cells. Doxorubicin‐loaded drug‐sponge NCs showed cytotoxicity in four cancer cell lines and capacity to inhibit tumor growth in subcutaneous xenografts of mice. Finally, unprecedented extraction of dye/drug cargos directly from cells and tissues (i.e. detoxification) was realized by the drug‐sponge NCs.