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Binding‐Mediated Formation of Ribonucleoprotein Corona for Efficient Delivery and Control of CRISPR/Cas9
Author(s) -
Wu Jinjun,
Peng Hanyong,
Lu Xiufen,
Lai Maode,
Zhang Hongquan,
Le X. Chris
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202014162
Subject(s) - ribonucleoprotein , crispr , cas9 , hela , corona (planetary geology) , hek 293 cells , microbiology and biotechnology , rna binding protein , gene delivery , rna , chemistry , computational biology , biology , nanotechnology , gene , cell , genetic enhancement , biochemistry , materials science , astrobiology , venus
Abstract Protein coronae formed with nanoparticles confer several useful properties. However, the non‐specific nature of protein corona formation makes it difficult to deliver specific proteins for therapeutic applications. Herein, we report on the construction of a new type of protein corona, termed binding‐mediated protein corona. This new corona enables the efficient and controllable delivery of functional proteins, which is otherwise challenging for conventional protein coronae. We show the design and delivery of the ribonucleoprotein corona for the CRISPR/Cas9 system. Successful gene editing in human cell lines (Hela and HEK293) demonstrates the efficient delivery, high stability, low cytotoxicity, and well‐controlled activity of the Cas9‐guide RNA ribonucleoprotein. The binding‐mediated protein corona strategy opens up new opportunities for therapeutic protein delivery.