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Cell‐Selective siRNA Delivery Using Glycosylated Dynamic Covalent Polymers Self‐Assembled In Situ by RNA Templating
Author(s) -
Laroui Nabila,
Coste Maëva,
Su Dandan,
Ali Lamiaa M. A.,
Bessin Yannick,
Barboiu Mihail,
GaryBobo Magali,
Bettache Nadir,
Ulrich Sébastien
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202014066
Subject(s) - covalent bond , cationic polymerization , polymer , bifunctional , polymerization , chemistry , in situ , combinatorial chemistry , nanotechnology , biophysics , materials science , polymer chemistry , biochemistry , organic chemistry , biology , catalysis
Dynamic covalent libraries enable exploring complex chemical systems from which bioactive assemblies can adaptively emerge through template effects. In this work, we studied dynamic covalent libraries made of complementary bifunctional cationic peptides, yielding a diversity of species from macrocycles to polymers. Although polymers are typically expressed only at high concentration, we found that siRNA acts as a template in the formation of dynamic covalent polymers at low concentration in a process guided by electrostatic binding. Using a glycosylated building block, we were able to show that this templated polymerization further translates into the multivalent presentation of carbohydrate ligands, which subsequently promotes cell uptake and even cell‐selective siRNA delivery.