Premium
NIR‐II pH Sensor with a FRET Adjustable Transition Point for In Situ Dynamic Tumor Microenvironment Visualization
Author(s) -
Zhao Mengyao,
Wang Jianbo,
Lei Zuhai,
Lu Lingfei,
Wang Shangfeng,
Zhang Hongxin,
Li Benhao,
Zhang Fan
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202012021
Subject(s) - chemistry , förster resonance energy transfer , near infrared spectroscopy , in situ , analytical chemistry (journal) , acceptor , wide dynamic range , dynamic range , in vivo , fluorescence , biophysics , chromatography , computer science , optics , physics , organic chemistry , biology , microbiology and biotechnology , computer vision , condensed matter physics
Monitoring the pH in tumor lesions provides abundant physiological information. However, currently developed pH sensors only achieve sensitive detection in the settled response region around the pH transition point (pH t ). To realize tumor pH monitoring with high sensitivity within a wider response region, reported here are serial pH t adjustable sensors (pTAS) that simply regulate the component ratio of second near‐infrared (NIR‐II) emission aza‐BODIPY (NAB) donor and pH sensitive rhodamine‐based pre‐acceptor (NRh) in Förster resonance energy transfer system. Combining the pH response regions of pTAS, a twofold widened pH detection range (6.11–7.22) is obtained compared to the pH t settled sensor (6.38–6.94). With an adjustable pH t , in vivo tumor pH increase and decrease processes could be dynamically visualized through dual‐channel ratiometric bioimaging within the NIR‐II window, with a coefficient of variation under 1 % compared to the standard pH meter.