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Magnetic‐Field‐Driven Extraction of Bioreceptors into Polymeric Membranes for Label‐Free Potentiometric Biosensing
Author(s) -
Lv Enguang,
Li Yanhong,
Ding Jiawang,
Qin Wei
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202011331
Subject(s) - potentiometric titration , membrane , biosensor , chemistry , extraction (chemistry) , nanotechnology , peptide , chromatography , combinatorial chemistry , biophysics , materials science , biochemistry , ion , biology , organic chemistry
We report here the concept of a magnetically controlled extraction of hydrophilic bioreceptors into polymeric membranes for bioassays. The potentiometric assay relies on the intrinsic charges of an antimicrobial peptide and its unique recognition abilities, which can eliminate the probe labeling and indicator addition. The target binding event could effectively prevent the extraction of the peptide into the polymeric membrane doped with an ion exchanger, thus resulting in a potential change. The potentiometric response properties of the peptide assembled on magnetic beads can be dynamically controlled and modulated by applying a magnetic field. Staphylococcus aureus, as a model of food‐borne pathogens, was measured at levels down to 10 CFU mL −1 . Based on this sensing strategy, a potentiometric array was developed for the pattern recognition of bacteria. The proposed general platform can be used for potentiometric biosensing using other hydrophilic bioreceptors.