Premium
A Cisplatin‐Selective Fluorescent Probe for Real‐Time Monitoring of Mitochondrial Platinum Accumulation in Living Cells
Author(s) -
Ong Jun Xiang,
Le Hai Van,
Lee Violet Eng Yee,
Ang Wee Han
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202010951
Subject(s) - mitochondrion , cisplatin , intracellular , microbiology and biotechnology , fluorescence , cell , chemistry , cancer cell , biophysics , biology , biochemistry , cancer , chemotherapy , physics , quantum mechanics , genetics
Mitochondria have emerged as important targets for cisplatin in cancer therapy. Apart from cisplatin, anticancer Pt complexes based on similar scaffolds have also been developed to target mitochondria. Yet cellular processing of cisplatin or these mitochondria‐targeting Pt analogues remained unexplored, largely due to a lack of tools capable of probing these Pt drugs within an intracellular environment. We developed the first mitochondria‐targeted fluorescent probe for real‐time monitoring of Pt accumulation in mitochondria. We applied the probe to investigate mitochondria as cellular targets for Pt drug complexes and uncovered two distinct pathways whereby these Pt complexes could be delivered to mitochondria after cell entry.