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Programmable Live‐Cell CRISPR Imaging with Toehold‐Switch‐Mediated Strand Displacement
Author(s) -
Hao Yaya,
Li Jiang,
Li Qian,
Zhang Luhao,
Shi Jiye,
Zhang Xueli,
Aldalbahi Ali,
Wang Lihua,
Fan Chunhai,
Wang Fei
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202009062
Subject(s) - crispr , cas9 , genome editing , synthetic biology , subgenomic mrna , genome engineering , computational biology , genome , dna , microbiology and biotechnology , biology , computer science , genetics , gene
The widespread application of CRISPR‐Cas9 has transformed genome engineering. Nevertheless, the precision to control the targeting activity of Cas9 requires further improvement. We report a toehold‐switch‐based approach to engineer the conformation of single guide RNA (sgRNA) for programmable activation of Cas9. This activation circuit is responsive to multiple inputs and can regulate the conformation of the sgRNA through toehold‐switch‐mediated strand displacement. We demonstrate the orthogonal suppression and activation of Cas9 with orthogonal DNA inputs. Combination of toehold switches leads to a variety of intracellular Cas9 activation programs with simultaneous and orthogonal responses, through which multiple genome loci are displayed in different colors in a controllable manner. This approach provides a new route for programing CRISPR in living cells for genome imaging and engineering.