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Amplified FRET Nanoflares: An Endogenous mRNA‐Powered Nanomachine for Intracellular MicroRNA Imaging
Author(s) -
Li Jing,
Wang Jiaoli,
Liu Shiyuan,
Xie Nuli,
Quan Ke,
Yang Yanjing,
Yang Xiaohai,
Huang Jin,
Wang Kemin
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202008245
Subject(s) - intracellular , förster resonance energy transfer , endogeny , microrna , messenger rna , molecular imaging , biophysics , microbiology and biotechnology , nanotechnology , chemistry , computational biology , biology , materials science , physics , fluorescence , biochemistry , gene , genetics , optics , in vivo
It is of great value to detect biological molecules in live cells. However, probes for imaging low‐abundance targets in live cells are limited by the one‐to‐one signal‐triggered model. Here, we introduce the concept of the amplified FRET nanoflare, which employs high‐abundance endogenous mRNA as fuel strands to amplify the detection of low abundance intracellular miRNA. As far as we know, this is the first report of an endogenous mRNA‐powered nanomachine for intracellular molecular detection. We experimentally prove the mechanism of the nanomachine and demonstrate its specificity and sensitivity. The proposed amplified FRET nanoflare can act as an excellent intracellular molecular detection strategy that is promising for biological and medical applications.