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Quantitative Protein Corona Composition and Dynamics on Carbon Nanotubes in Biological Environments
Author(s) -
Pinals Rebecca L.,
Yang Darwin,
Rosenberg Daniel J.,
Chaudhary Tanya,
Crothers Andrew R.,
Iavarone Anthony T.,
Hammel Michal,
Landry Markita P.
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202008175
Subject(s) - carbon nanotube , biophysics , corona (planetary geology) , chemistry , nanoparticle , nanotoxicology , nanotechnology , protein adsorption , adsorption , materials science , biology , organic chemistry , astrobiology , venus
When nanoparticles enter biological environments, proteins adsorb to form the “protein corona” which alters nanoparticle biodistribution and toxicity. Herein, we measure protein corona formation on DNA‐functionalized single‐walled carbon nanotubes (ssDNA‐SWCNTs), a nanoparticle used widely for sensing and delivery, in blood plasma and cerebrospinal fluid. We characterize corona composition by mass spectrometry, revealing high‐abundance corona proteins involved in lipid binding, complement activation, and coagulation. We investigate roles of electrostatic and entropic interactions driving selective corona formation. Lastly, we study real‐time protein binding on ssDNA‐SWCNTs, obtaining agreement between enriched proteins binding strongly and depleted proteins binding marginally, while highlighting cooperative adsorption mechanisms. Knowledge of protein corona composition, formation mechanisms, and dynamics informs nanoparticle translation from in vitro design to in vivo application.