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Cross‐Link‐Functionalized Nanoparticles for Rapid Excretion in Nanotheranostic Applications
Author(s) -
Ma Zhuoran,
Wang Feifei,
Zhong Yeteng,
Salazar Felix,
Li Jiachen,
Zhang Mingxi,
Ren Fuqiang,
Wu Anna M.,
Dai Hongjie
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202008083
Subject(s) - biocompatibility , lead sulfide , nanoparticle , in vivo , chemistry , amphiphile , cadmium sulfide , nanotechnology , nanomedicine , coating , materials science , polymer , biophysics , quantum dot , inorganic chemistry , microbiology and biotechnology , organic chemistry , copolymer , biology
Most NIR‐IIb fluorophores are nanoparticle‐based probes with long retention (≈1 month or longer) in the body. Here, we applied a novel cross‐linked coating to functionalize core/shell lead sulfide/cadmium sulfide quantum dots (PbS/CdS QDs) emitting at ≈1600 nm. The coating was comprised of an amphiphilic polymer followed by three crosslinked amphiphilic polymeric layers (P 3 coating), imparting high biocompatibility and >90 % excretion of QDs within 2 weeks of intravenous administration. The P 3 ‐QDs were conjugated to an engineered anti‐CD8 diabody (Cys‐diabody) for in vivo molecular imaging of CD8+ cytotoxic T lymphocytes (CTLs) in response to anti‐PD‐L1 therapy. Two‐plex molecular imaging in combination with down‐conversion Er nanoparticles (ErNPs) was performed for real‐time in vivo monitoring of PD‐L1 positive tumor cells and CTLs with cellular resolution by non‐invasive NIR‐IIb light sheet microscopy. Imaging of angiogenesis in the tumor microenvironment and of lymph nodes deep in the body with a signal‐to‐background ratio of up to ≈170 was also achieved using P 3 ‐QDs.