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Metal–Organic Framework Disintegrants: Enzyme Preparation Platforms with Boosted Activity
Author(s) -
An Hongde,
Song Jie,
Wang Ting,
Xiao Nannan,
Zhang Zhenjie,
Cheng Peng,
Ma Shengqian,
Huang He,
Chen Yao
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202007827
Subject(s) - biocatalysis , enzyme , chemistry , metal ions in aqueous solution , immobilized enzyme , combinatorial chemistry , homogeneous , metal , activator (genetics) , enzyme assay , materials science , nanotechnology , biochemistry , organic chemistry , catalysis , reaction mechanism , receptor , physics , thermodynamics
An enzyme formulation using customized enzyme activators (metal ions) to directly construct metal–organic frameworks (MOFs) as enzyme protective carriers is presented. These MOF carriers can also serve as the disintegrating agents to simultaneously release enzymes and their activators during biocatalysis with boosted activities. This highly efficient enzyme preparation combines enzyme immobilization (enhanced stability, easy operation) and homogeneous biocatalysis (fast diffusion, high activity). The MOF serves as an ion pump that continuously provides metal ion activators that greatly promote the enzymatic activities (up to 251 %). This MOF–enzyme composite demonstrated an excellent protective effect against various perturbation environments. A mechanistic investigation revealed that the spontaneous activator/enzyme release and ion pumping enable enzymes to sufficiently interact with their activators owing to the proximity effects, leading to a boost in biocatalytic performance.