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Surface‐Plasmonic‐Field‐Induced Photoredox Catalysis and Mediated Electron Transfer for Washing‐Free DNA Detection
Author(s) -
Kim Jihyeon,
Park Jongkyoon,
Park Seonhwa,
Seo Jeongwook,
Kwon Jeongwook,
Lee Hyunsoo,
Kim Seungchul,
Yang Haesik
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202007318
Subject(s) - electron transfer , triethanolamine , photochemistry , chemistry , catalysis , quenching (fluorescence) , photoredox catalysis , detection limit , fluorescence , photoinduced electron transfer , electrochemistry , electrode , photocatalysis , analytical chemistry (journal) , organic chemistry , chromatography , optics , physics
Distance‐dependent electromagnetic radiation and electron transfer have been commonly employed in washing‐free fluorescence and electrochemical bioassays, respectively. In this study, we combined the two distance‐dependent phenomena for sensitive washing‐free DNA detection. A distance‐dependent surface plasmonic field induces rapid photoredox catalysis of surface‐bound catalytic labels, and distance‐dependent mediated electron transfer allows for rapid electron transfer from the surface‐bound labels to the electrode. An optimal system consists of a chemically reversible acceptor (Ru(NH 3 ) 6 3+ ), a chemically reversible photoredox catalyst (eosin Y), and a chemically irreversible donor (triethanolamine). Side reactions with O 2 do not significantly decrease the efficiency of photoredox catalysis. Energy transfer quenching between the electrode and the label can be lowered by increasing the distance between them. Washing‐free DNA detection had a detection limit of approximately 0.3 n m in buffer and 0.4 n m in serum without a washing step.