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A Far‐Red Emitting Fluorescent Chemogenetic Reporter for In Vivo Molecular Imaging
Author(s) -
Li Chenge,
Tebo Alison G.,
Thauvin Marion,
Plamont MarieAude,
Volovitch Michel,
Morin Xavier,
Vriz Sophie,
Gautier Arnaud
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202006576
Subject(s) - fluorescence , far red , green fluorescent protein , rhodanine , fluorescent protein , zebrafish , in vivo , chemistry , preclinical imaging , biophysics , biology , biochemistry , gene , genetics , optics , red light , botany , physics
Far‐red emitting fluorescent labels are highly desirable for spectral multiplexing and deep tissue imaging. Here, we describe the generation of frFAST (far‐red Fluorescence Activating and absorption Shifting Tag), a 14‐kDa monomeric protein that forms a bright far‐red fluorescent assembly with (4‐hydroxy‐3‐methoxy‐phenyl)allylidene rhodanine (HPAR‐3OM). As HPAR‐3OM is essentially non‐fluorescent in solution and in cells, frFAST can be imaged with high contrast in presence of free HPAR‐3OM, which allowed the rapid and efficient imaging of frFAST fusions in live cells, zebrafish embryo/larvae, and chicken embryos. Beyond enabling the genetic encoding of far‐red fluorescence, frFAST allowed the design of a far‐red chemogenetic reporter of protein–protein interactions, demonstrating its great potential for the design of innovative far‐red emitting biosensors.