Premium
An Integrated Microfluidic Probe for Mass Spectrometry Imaging of Biological Samples **
Author(s) -
Li Xiangtang,
Yin Ruichuan,
Hu Hang,
Li Yingju,
Sun Xiaofei,
Dey Sudhansu K.,
Laskin Julia
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202006531
Subject(s) - mass spectrometry , desorption electrospray ionization , mass spectrometry imaging , microfluidics , nanotechnology , nano , analytical chemistry (journal) , materials science , chemistry , ionization , chromatography , chemical ionization , ion , organic chemistry , composite material
Ambient ionization based on liquid extraction is widely used in mass spectrometry imaging (MSI) of molecules in biological samples. The development of nanospray desorption electrospray ionization (nano‐DESI) has enabled the robust imaging of tissue sections with high spatial resolution. However, the fabrication of the nano‐DESI probe is challenging, which limits its dissemination to the broader scientific community. Herein, we describe the design and performance of an integrated microfluidic probe (iMFP) for nano‐DESI MSI. The glass iMFP, fabricated using photolithography, wet etching, and polishing, shows comparable performance to the capillary‐based nano‐DESI MSI in terms of stability and sensitivity; a spatial resolution of better than 25 μm was obtained in these first proof‐of‐principle experiments. The iMFP is easy to operate and align in front of a mass spectrometer, which will facilitate broader use of liquid‐extraction‐based MSI in biological research, drug discovery, and clinical studies.