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Dual‐Mode Superresolution Imaging Using Charge Transfer Dynamics in Semiconducting Polymer Dots
Author(s) -
Jiang Yifei,
Hu Qiongzheng,
Chen Haobin,
Zhang Jicheng,
Chiu Daniel T.,
McNeill Jason
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202006348
Subject(s) - materials science , polymer , fluorescence lifetime imaging microscopy , optoelectronics , temporal resolution , nanoparticle , population , superresolution , biological imaging , nanotechnology , fluorescence , optics , physics , computer science , image (mathematics) , artificial intelligence , composite material , demography , sociology
In a conjugated polymer‐based single‐particle heterojunction, stochastic fluctuations of the photogenerated hole population lead to spontaneous fluorescence switching. We found that 405 nm irradiation can induce charge recombination and activate the single‐particle emission. Based on these phenomena, we developed a novel class of semiconducting polymer dots that can operate in two superresolution imaging modes. The spontaneous switching mode offers efficient imaging of large areas, with <10 nm localization precision, while the photoactivation/deactivation mode offers slower imaging, with further improved localization precision (ca. 1 nm), showing advantages in resolving small structures that require high spatial resolution. Superresolution imaging of microtubules and clathrin‐coated pits was demonstrated, under both modes. The excellent localization precision and versatile imaging options provided by these nanoparticles offer clear advantages for imaging of various biological systems.