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A Robust Au−C≡C Functionalized Surface: Toward Real‐Time Mapping and Accurate Quantification of Fe 2+ in the Brains of Live AD Mouse Models
Author(s) -
Zhang Chuanping,
Liu Zhichao,
Zhang Limin,
Zhu Anwei,
Liao Fumin,
Wan Jingjing,
Zhou Jian,
Tian Yang
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202006318
Subject(s) - microelectrode , striatum , multielectrode array , electrochemistry , chemistry , biocompatibility , biosensor , avidity , biophysics , neuroscience , biochemistry , electrode , biology , antibody , immunology , organic chemistry , dopamine
Described here is that Au−C≡C bonds showed the highest stability under biological conditions, with abundant thiols, and the best electrochemical performance compared to Au−S and Au−Se bonds. The new finding was also confirmed by theorical calculations. Based on this finding, a specific molecule for recognition of Fe 2+ was designed and synthesized, and used to create a selective and accurate electrochemical sensor for the quantification of Fe 2+ . The present ratiometric strategy demonstrates high spatial resolution for real‐time tracking of Fe 2+ in a dynamic range of 0.2–120 μM. Finally, a microelectrode array with good biocompatibility was applied in imaging and biosensing of Fe 2+ in the different regions of live mouse brains. Using this tool, it was discovered that the uptake of extracellular Fe 2+ into the cortex and striatum was largely mediated by cyclic adenosine monophosphate (cAMP) through the CREB‐related pathway in the brain of a mouse with Alzheimer's disease.