Premium
Back Cover: In Vitro Selection of a DNA Aptamer Targeting Degraded Protein Fragments for Biosensing (Angew. Chem. Int. Ed. 20/2020)
Author(s) -
Liu Meng,
Wang Jiayi,
Chang Yangyang,
Zhang Qiang,
Chang Dingran,
Hui Christy Y.,
Brennan John D.,
Li Yingfu
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202004665
Subject(s) - aptamer , systematic evolution of ligands by exponential enrichment , dna , chemistry , in vitro , biosensor , computational biology , cover (algebra) , microbiology and biotechnology , protein detection , target protein , biochemistry , biology , nanotechnology , gene , rna , materials science , mechanical engineering , engineering
A DNA aptamer targeting naturally degraded protein fragments was selected by M. Liu, J. D. Brennan, Y. Li, and co‐workers in their Communication on page 7706. The aptamer binds a degraded form of toxin B from Clostridium difficile and can detect this protein fragment in human stool. This method offers a way to discover DNA aptamers that cannot be selected using purified proteins, thus expanding the practical utility of DNA aptamers in clinical diagnosis.