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An Electrochemophysiological Microarray for Real‐Time Monitoring and Quantification of Multiple Ions in the Brain of a Freely Moving Rat
Author(s) -
Zhao Fan,
Liu Yuandong,
Dong Hui,
Feng Shiqing,
Shi Guoyue,
Lin Longnian,
Tian Yang
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202002417
Subject(s) - microelectrode , ion , electrode , chemistry , biosensor , analytical chemistry (journal) , computer science , materials science , nanotechnology , chromatography , organic chemistry
Herein, we present an electrochemophysiological microarray (ECPM) for real‐time mapping and simultaneous quantification of chemical signals for multiple ions in the deep brain of a freely moving rat, in which microelectrode arrays were developed for direct determination of multiple ions using open‐circuit potentiometry. Specific recognition ionophores were synthesized and optimized for determination of K + , Ca 2+ , Na + and pH. A reference electrode was also developed to avoid interferences in the brain. The microarrays were successfully applied in real‐time monitoring and quantification of ions in a live brain. The extra current‐free potentiometry allowed mapping and biosensing of chemical signals, together with recording of electrical signals in the whole brain without cross‐talk, for the first time. Furthermore, the ECPM provided a platform for real‐time monitoring of the dynamic changes of multiple ions in the deep brain of freely moving rat during a seizure.