Premium
Efficient Chemical Protein Synthesis using Fmoc‐Masked N‐Terminal Cysteine in Peptide Thioester Segments
Author(s) -
Kar Abhisek,
Mannuthodikayil Jamsad,
Singh Sameer,
Biswas Anamika,
Dubey Puneet,
Das Amit,
Mandal Kalyaneswar
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202000491
Subject(s) - native chemical ligation , chemistry , thioester , chemical ligation , peptide , cysteine , piperidine , peptide synthesis , moiety , combinatorial chemistry , residue (chemistry) , chemical synthesis , hydrazide , solid phase synthesis , protecting group , organic chemistry , biochemistry , in vitro , enzyme , alkyl
Abstract We report an operationally simple method to facilitate chemical protein synthesis by fully convergent and one‐pot native chemical ligations utilizing the fluorenylmethyloxycarbonyl (Fmoc) moiety as an N‐masking group of the N‐terminal cysteine of the middle peptide thioester segment(s). The Fmoc group is stable to the harsh oxidative conditions frequently used to generate peptide thioesters from peptide hydrazide or o ‐aminoanilide. The ready availability of Fmoc‐Cys(Trt)‐OH, which is routinely used in Fmoc solid‐phase peptide synthesis, where the Fmoc group is pre‐installed on cysteine residue, minimizes additional steps required for the temporary protection of the N‐terminal cysteinyl peptides. The Fmoc group is readily removed after ligation by short exposure (<7 min) to 20 % piperidine at pH 11 in aqueous conditions at room temperature. Subsequent native chemical ligation reactions can be performed in presence of piperidine in the same solution at pH 7.