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Rational Design of a Two‐Photon Fluorogenic Probe for Visualizing Monoamine Oxidase A Activity in Human Glioma Tissues
Author(s) -
Fang Haixiao,
Zhang Hang,
Li Lin,
Ni Yun,
Shi Riri,
Li Zheng,
Yang Xuekang,
Ma Bo,
Zhang Chengwu,
Wu Qiong,
Yu Changmin,
Yang Naidi,
Yao Shao Q.,
Huang Wei
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202000059
Subject(s) - monoamine oxidase , glioma , monoamine neurotransmitter , monoamine oxidase b , fluorescence , gene isoform , monoamine oxidase a , enzyme , human brain , two photon excitation microscopy , biochemistry , fluorescence microscope , cytotoxicity , chemistry , endogeny , biology , computational biology , neuroscience , in vitro , cancer research , gene , serotonin , physics , receptor , quantum mechanics
Abstract Monoamine oxidases have two functionally distinct but structurally similar isoforms (MAO‐A and MAO‐B). The ability to differentiate them by using fluorescence detection/imaging technology is of significant biological relevance, but highly challenging with available chemical tools. Herein, we report the first MAO‐A‐specific two‐photon fluorogenic probe ( F1 ), capable of selective imaging of endogenous MAO‐A enzymatic activities from a variety of biological samples, including MAO‐A‐expressing neuronal SY‐SY5Y cells, the brain of tumor‐bearing mice and human Glioma tissues by using two‐photon fluorescence microscopy (TPFM) with minimal cytotoxicity.