Premium
Mirror‐Image 5S Ribonucleoprotein Complexes
Author(s) -
Ling JunJie,
Fan Chuyao,
Qin Hong,
Wang Min,
Chen Ji,
WittungStafshede Pernilla,
Zhu Ting F.
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201914799
Subject(s) - ribonucleoprotein , ribosome , ribosomal rna , rna , ribosomal protein , transcription (linguistics) , translation (biology) , biology , computational biology , genetics , microbiology and biotechnology , gene , messenger rna , linguistics , philosophy
After realizing mirror‐image genetic replication, transcription, and reverse transcription, the biggest challenge in establishing a mirror‐image version of the central dogma is to build a mirror‐image ribosome‐based translation machine. Here, we chemically synthesized the natural and mirror‐image versions of three ribosomal proteins (L5, L18, and L25) in the large subunit of the Escherichia coli ribosome with post‐translational modifications. We show that the synthetic mirror‐image proteins can fold in vitro despite limited efficiency and assemble with enzymatically transcribed mirror‐image 5S ribosomal RNA into ribonucleoprotein complexes. In addition, the RNA–protein interactions are chiral‐specific in that the mirror‐image ribosomal proteins do not bind with natural 5S ribosomal RNA and vice versa. The synthesis and assembly of mirror‐image 5S ribonucleoprotein complexes are important steps towards building a functional mirror‐image ribosome.