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Radical Transfer Dissociation for De Novo Characterization of Modified Ribonucleic Acids by Mass Spectrometry
Author(s) -
Calderisi Giovanni,
Glasner Heidelinde,
Breuker Kathrin
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201914275
Subject(s) - rna , mass spectrometry , dissociation (chemistry) , chemistry , transfer rna , electron transfer dissociation , nucleotide , combinatorial chemistry , computational biology , chromatography , biochemistry , tandem mass spectrometry , biology , organic chemistry , gene
Abstract Mass spectrometry (MS) can reliably detect and localize all mass‐altering modifications of ribonucleic acids (RNA), but current MS approaches that allow for simultaneous de novo sequencing and modification analysis generally require specialized instrumentation. Here we report a novel RNA dissociation technique, radical transfer dissociation (RTD), that can be used for the comprehensive de novo characterization of ribonucleic acids and their posttranscriptional or synthetic modifications. We demonstrate full sequence coverage for RNA consisting of up to 39 nucleotides and show that RTD is especially useful for RNA with highly labile modifications such as 5‐hydroxymethylcytidine and 5‐formylcytidine.