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Amphiphilic Polyphenylene Dendron Conjugates for Surface Remodeling of Adenovirus 5
Author(s) -
Wagner Jessica,
Li Longjie,
Simon Johanna,
Krutzke Lea,
Landfester Katharina,
Mailänder Volker,
Müllen Klaus,
Ng David Y. W.,
Wu Yuzhou,
Weil Tanja
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201913708
Subject(s) - dendrimer , amphiphile , surface modification , chemistry , transduction (biophysics) , gene delivery , conjugate , biophysics , combinatorial chemistry , copolymer , genetic enhancement , gene , polymer chemistry , biochemistry , organic chemistry , polymer , biology , mathematical analysis , mathematics
Abstract Amphiphilic surface groups play an important role in many biological processes. The synthesis of amphiphilic polyphenylene dendrimer branches (dendrons), providing alternating hydrophilic and lipophilic surface groups and one reactive ethynyl group at the core is reported. The amphiphilic surface groups serve as biorecognition units that bind to the surface of adenovirus 5 (Ad5), which is a common vector in gene therapy. The Ad5/dendron complexes showed high gene transduction efficiencies in coxsackie‐adenovirus receptor (CAR)‐negative cells. Moreover, the dendrons offer incorporation of new functions at the dendron core by in situ post‐modifications, even when bound to the Ad5 surface. Surfaces coated with these dendrons were analyzed for their blood‐protein binding capacity, which is essential to predict their performance in the blood stream. A new platform for introducing bioactive groups to the Ad5 surface without chemically modifying the virus particles is provided.