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Modulating the Biofunctionality of Metal–Organic‐Framework‐Encapsulated Enzymes through Controllable Embedding Patterns
Author(s) -
Chen Guosheng,
Kou Xiaoxue,
Huang Siming,
Tong Linjing,
Shen Yujian,
Zhu Wangshu,
Zhu Fang,
Ouyang Gangfeng
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201913231
Subject(s) - enzyme , embedding , chemistry , nucleation , metal organic framework , materials science , nanotechnology , biochemistry , computer science , organic chemistry , adsorption , artificial intelligence
Embedding an enzyme within a MOF as exoskeleton (enzyme@MOF) offers new opportunities to improve the inherent fragile nature of the enzyme, but also to impart novel biofunctionality to the MOF. Despite the remarkable stability achieved for MOF‐embedded enzymes, embedding patterns and conversion of the enzymatic biofunctionality after entrapment by a MOF have only received limited attention. Herein, we reveal how embedding patterns affect the bioactivity of an enzyme encapsulated in ZIF‐8. The enzyme@MOF can maintain high activity when the encapsulation process is driven by rapid enzyme‐triggered nucleation of ZIF‐8. When the encapsulation is driven by slow coprecipitation and the enzymes are not involved in the nucleation of ZIF‐8, enzyme@MOF tends to be inactive owing to unfolding and competing coordination caused by the ligand, 2‐methyl imidazole. These two embedding patterns can easily be controlled by chemical modification of the amino acids of the enzymes, modulating their biofunctionality.