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Discriminative Detection of Biothiols by Electron Paramagnetic Resonance Spectroscopy using a Methanethiosulfonate Trityl Probe
Author(s) -
Tan Xiaoli,
Ji Kaiyun,
Wang Xing,
Yao Ru,
Han Guifang,
Villamena Frederick A.,
Zweier Jay L.,
Song Yuguang,
Rockenbauer Antal,
Liu Yangping
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201912832
Subject(s) - chemistry , electron paramagnetic resonance , glutathione , cysteine , thiol , buthionine sulfoximine , conjugate , dithiocarbamate , photochemistry , electron paramagnetic resonance spectroscopy , nuclear magnetic resonance , organic chemistry , mathematical analysis , physics , mathematics , enzyme
Biothiols, such as glutathione (GSH), homocysteine (Hcy), and cysteine (Cys), coexist in biological systems with diverse biological roles. Thus, analytical techniques that can detect, quantify, and distinguish between multiple biothiols are desirable but challenging. Herein, we demonstrate the simultaneous detection and quantitation of multiple biothiols, including up to three different biothiols in a single sample, using electron paramagnetic resonance (EPR) spectroscopy and a trityl‐radical‐based probe (MTST). We term this technique EPR thiol‐trapping. MTST could trap thiols through its methanethiosulfonate group to form the corresponding disulfide conjugate with an EPR spectrum characteristic of the trapped thiol. MTST was used to investigate effects of l ‐buthionine sulfoximine (BSO) and pyrrolidine dithiocarbamate (PDTC) on the efflux of GSH and Cys from HepG2 cells.