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Genetically Encoded Ratiometric RNA‐Based Sensors for Quantitative Imaging of Small Molecules in Living Cells
Author(s) -
Wu Rigumula,
Karunanayake Mudiyanselage Aruni P. K. K.,
Shafiei Fatemeh,
Zhao Bin,
Bagheri Yousef,
Yu Qikun,
McAuliffe Kathleen,
Ren Kewei,
You Mingxu
Publication year - 2019
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201911799
Subject(s) - aptamer , rna , intracellular , chemistry , cell , small molecule , fluorescence , cell signaling , live cell imaging , molecule , förster resonance energy transfer , microbiology and biotechnology , biophysics , computational biology , nanotechnology , biochemistry , biology , signal transduction , gene , materials science , physics , organic chemistry , quantum mechanics
Precisely determining the intracellular concentrations of metabolites and signaling molecules is critical in studying cell biology. Fluorogenic RNA‐based sensors have emerged to detect various targets in living cells. However, it is still challenging to apply these genetically encoded sensors to quantify the cellular concentrations and distributions of targets. Herein, using a pair of orthogonal fluorogenic RNA aptamers, DNB and Broccoli, we engineered a modular sensor system to apply the DNB‐to‐Broccoli fluorescence ratio to quantify the cell‐to‐cell variations of target concentrations. These ratiometric sensors can be broadly applied for live‐cell imaging and quantification of metabolites, signaling molecules, and other synthetic compounds.