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Tracking Pathogen Infections by Time‐Resolved Chemical Proteomics
Author(s) -
Zhang Ying,
Kao DerShyang,
Gu Bing,
Bomjan Rajdeep,
Srivastava Mayank,
Lu Haojie,
Zhou Daoguo,
Tao W. Andy
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201911078
Subject(s) - proteomics , pathogen , host–pathogen interaction , proteome , computational biology , host (biology) , biology , vacuole , quantitative proteomics , chemistry , microbiology and biotechnology , nanotechnology , biophysics , biochemistry , virulence , gene , materials science , genetics , cytoplasm
Studying the dynamic interaction between host cells and pathogen is vital but remains technically challenging. We describe herein a time‐resolved chemical proteomics strategy enabling host and pathogen temporal interaction profiling (HAPTIP) for tracking the entry of a pathogen into the host cell. A novel multifunctional chemical proteomics probe was introduced to label living bacteria followed by in vivo crosslinking of bacteria proteins to their interacting host‐cell proteins at different time points initiated by UV for label‐free quantitative proteomics analysis. We observed over 400 specific interacting proteins crosslinked with the probe during the formation of Salmonella ‐containing vacuole (SCV). This novel chemical proteomics approach provides a temporal interaction profile of host and pathogen in high throughput and would facilitate better understanding of the infection process at the molecular level.