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Orthogonal Activation of RNA‐Cleaving DNAzymes in Live Cells by Reactive Oxygen Species
Author(s) -
Xiao Lu,
Gu Chunmei,
Xiang Yu
Publication year - 2019
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201908105
Subject(s) - deoxyribozyme , reactive oxygen species , chemistry , hydrogen peroxide , oxidative stress , intracellular , microbiology and biotechnology , rna , biophysics , nanotechnology , biochemistry , dna , biology , gene , materials science
RNA‐cleaving DNAzymes are useful tools for intracellular metal‐ion sensing and gene regulation. Incorporating stimuli‐responsive modifications into these DNAzymes enables their activities to be spatiotemporally and chemically controlled for more precise applications. Despite the successful development of many caged DNAzymes for light‐induced activation, DNAzymes that can be intracellularly activated by chemical inputs of biological importance, such as reactive oxygen species (ROS), are still scarce. ROS like hydrogen peroxide (H 2 O 2 ) and hypochlorite (HClO) are critical mediators of oxidative stress‐related cell signaling and dysregulation including activation of immune system as well as progression of diseases and aging. Herein, we report ROS‐activable DNAzymes by introducing phenylboronate and phosphorothioate modifications to the Zn 2+ ‐dependent 8–17 DNAzyme. These ROS‐activable DNAzymes were orthogonally activated by H 2 O 2 and HClO inside live human and mouse cells.