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Fast and Quantitative NMR Metabolite Analysis Afforded by a Paramagnetic Co‐Solute
Author(s) -
Mulder Frans A. A.,
Tenori Leonardo,
Luchinat Claudio
Publication year - 2019
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201908006
Subject(s) - paramagnetism , nuclear magnetic resonance spectroscopy , chemistry , spectroscopy , gadolinium , spins , relaxation (psychology) , transverse relaxation optimized spectroscopy , metabolomics , spin–lattice relaxation , robustness (evolution) , two dimensional nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , chemical physics , nuclear magnetic resonance , fluorine 19 nmr , physics , chromatography , stereochemistry , organic chemistry , condensed matter physics , psychology , social psychology , quantum mechanics , biochemistry , gene
NMR spectroscopy is an indispensable technique for the determination of the chemical identity and structure of small molecules. The method is especially recognized for its robustness and intrinsically quantitative nature, and has manifested itself as a key analytical platform for diverse fields of application, ranging from chemical synthesis to metabolomics. Unfortunately, the slow recovery of nuclear spin polarization by spin‐lattice (T 1 ) relaxation causes most experimental time to be lost on idle waiting. Furthermore, truly quantitative NMR (qNMR) spectroscopy requires waiting times of 5‐times the longest T 1 in the sample, making qNMR spectroscopy slow and inefficient. We demonstrate here that co‐solute paramagnetic relaxation can mitigate these two problems simultaneously. The addition of a small amount of paramagnetic gadolinium chelate, available in the form of commercial contrast‐agent solutions, enables cheap, quantitative, and efficient high‐throughput mixture analysis.