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Visualizing Microglia with a Fluorescence Turn‐On Ugt1a7c Substrate
Author(s) -
Kim Beomsue,
Fukuda Masahiro,
Lee JungYeol,
Su Dongdong,
Sanu Srikanta,
Silvin Aymeric,
Khoo Audrey T. T.,
Kwon Taejoon,
Liu Xiao,
Chi Weijie,
Liu Xiaogang,
Choi Sejong,
Wan Diana S. Y.,
Park SungJin,
Kim JinSoo,
Ginhoux Florent,
Je H. Shawn,
Chang YoungTae
Publication year - 2019
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201903058
Subject(s) - turn (biochemistry) , microglia , fluorescence , substrate (aquarium) , chemistry , biophysics , biology , biochemistry , physics , optics , ecology , immunology , inflammation
Microglia, the brain‐resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure–activity relationships study, we developed CDr20, a high‐performance fluorogenic chemical probe that enables the visualization of microglia both in vitro and in vivo. Using a genome‐scale CRISPR‐Cas9 knockout screen, the UDP‐glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence.