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Diterpene Biosynthesis in Actinomycetes: Studies on Cattleyene Synthase and Phomopsene Synthase
Author(s) -
Rinkel Jan,
Steiner Simon T.,
Dickschat Jeroen S.
Publication year - 2019
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201902950
Subject(s) - diterpene , nocardia , atp synthase , biosynthesis , enzyme , streptomyces , polyketide synthase , biochemistry , chemistry , actinomycetales , stereochemistry , fragmentation (computing) , biology , bacteria , polyketide , genetics , ecology
Three diterpene synthases from actinomycetes have been studied. The first enzyme from Streptomyces cattleya produced the novel compound cattleyene. The other two enzymes from Nocardia testacea and Nocardia rhamnosiphila were identified as phomopsene synthases. The cyclisation mechanism of cattleyene synthase and the EIMS fragmentation mechanism of its product were extensively studied by incubation experiments with isotopically labelled precursors. Oxidative transformations expanded the chemical space of these unique diterpenes.