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Bacteria‐Based Production of Thiol‐Clickable, Genetically Encoded Lipid Nanovesicles
Author(s) -
Royes Jorge,
Ilioaia Oana,
Lubart Quentin,
Angius Federica,
Dubacheva Galina V.,
Bally Marta,
Miroux Bruno,
Tribet Christophe
Publication year - 2019
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201902929
Subject(s) - dispersity , maleimide , chemistry , click chemistry , surface modification , liposome , thiol , vesicle , transmembrane protein , combinatorial chemistry , biophysics , nanotechnology , membrane , materials science , biochemistry , organic chemistry , biology , receptor
Despite growing research efforts on the preparation of (bio)functional liposomes, synthetic capsules cannot reach the densities of protein loading and the control over peptide display that is achieved by natural vesicles. Herein, a microbial platform for high‐yield production of lipidic nanovesicles with clickable thiol moieties in their outer corona is reported. These nanovesicles show low size dispersity, are decorated with a dense, perfectly oriented, and customizable corona of transmembrane polypeptides. Furthermore, this approach enables encapsulation of soluble proteins into the nanovesicles. Due to the mild preparation and loading conditions (absence of organic solvents, pH gradients, or detergents) and their straightforward surface functionalization, which takes advantage of the diversity of commercially available maleimide derivatives, bacteria‐based proteoliposomes are an attractive eco‐friendly alternative that can outperform currently used liposomes.