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Mitochondria‐Targeting, Intracellular Delivery of Native Proteins Using Biodegradable Silica Nanoparticles
Author(s) -
Yuan Peiyan,
Mao Xin,
Wu Xiaofeng,
Liew Si Si,
Li Lin,
Yao Shao Q.
Publication year - 2019
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201901699
Subject(s) - mitochondrion , intracellular , organelle , chemistry , drug delivery , biophysics , biochemistry , microbiology and biotechnology , biology , organic chemistry
Mitochondria are key organelles in mammalian cells whose dysfunction is linked to various diseases. Drugs targeting mitochondrial proteins provide a highly promising strategy for potential therapeutics. Methods for the delivery of small‐molecule drugs to the mitochondria are available, but these are not suitable for macromolecules, such as proteins. Herein, we report the delivery of native proteins and antibodies to the mitochondria using biodegradable silica nanoparticles (BS–NPs). The modification of the nanoparticle surface with triphenylphosphonium (TPP) and cell‐penetrating poly(disulfide)s (CPD) facilitated their rapid intracellular uptake with minimal endolysosomal trapping, providing sufficient time for effective mitochondrial localization followed by glutathione‐triggered biodegradation and of native, functional proteins into the mitochondria.