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Inside Cover: EPR Spectroscopy Detects Various Active State Conformations of the Transcriptional Regulator CueR (Angew. Chem. Int. Ed. 10/2019)
Author(s) -
Sameach Hila,
Ghosh Shreya,
GevorkyanAirapetov Lada,
Saxena Sunil,
Ruthstein Sharon
Publication year - 2019
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201900968
Subject(s) - dna , chemistry , electron paramagnetic resonance , site directed spin labeling , regulator , transcription (linguistics) , crystallography , transcriptional regulation , spectroscopy , biophysics , ion , stereochemistry , transcription factor , gene , biochemistry , nuclear magnetic resonance , biology , physics , linguistics , philosophy , organic chemistry , quantum mechanics
Cu II ‐‐NTA spin labeling of the transcription regulator CueR, an E . coli copper‐sensing protein, enabled the high‐resolution detection of its various conformational states, as described by S. Ruthstein and co‐workers in their Communication on page 3053 ff. Two of the conformations, activators 1 and 2, represent CueR as it binds DNA and Cu I ions. The computed structures suggest that one is more compressed on the DNA than the other and the transition between those states depends on Cu I concentration in the bacterial cell.