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Freezing‐directed Stretching and Alignment of DNA Oligonucleotides
Author(s) -
Liu Biwu,
Wu Tianyi,
Huang Zhicheng,
Liu Yibo,
Liu Juewen
Publication year - 2019
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201814352
Subject(s) - oligonucleotide , dna , förster resonance energy transfer , biophysics , fluorescence , chemistry , raman spectroscopy , nanomaterials , materials science , nanotechnology , biochemistry , biology , physics , quantum mechanics , optics
Most single‐stranded DNA oligonucleotides are random coils with a persistence length of below 1 nm. So far, no good methods are available to stretch oligonucleotides. Herein, it is shown that freezing can stretch DNA, as confirmed using fluorescence resonance energy transfer, thiazole‐orange staining, and surface‐enhanced Raman spectroscopy. Lateral inter‐strand interactions are critical, and the stretched DNA oligonucleotides are aligned. This work also provides a set of methods for studying frozen oligonucleotides. Upon freezing, DNA oligonucleotides are readily adsorbed onto various nanomaterials, including gold nanoparticles, graphene oxide, iron oxide, and WS 2 via the most thermodynamically stable conformation, leading to more stable conjugates.